Reporter

Part:BBa_K2150022:Design

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pTet+RBS+T7RNAP+Ter+pT7+RBS+GFP+DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3488


Design Notes

Two different terminators are used to avoid repeated sequence.

Source

T7RNAP:PCR amplified from genome of BL21.

First terminator comes from BBa_B0013, while the second terminator from BBa_B0015. PTet from BBa_R0040,RBS from BBa_B0034, pT7 from BBa_K525998, GFP from BBa_E0040.

References